Overview

Designed as an introductory text the authors cover all core strategies in the application of modern recombinant DNA technology. The first chapters directly address the applications of polymerase chain reaction to a variety of problems in DNA cloning that are, or have been, extremely challenging using more traditional approaches and technologies. These include cDNA cloning and transcript mapping, mutagenesis as well as the cloning of very long transcripts and protocols using limiting amounts of total RNA. Further chapters describe approaches to subtractive cloning technologies as well as novel specialized expression cloning and library screening strategies. The handbook contains detailed step-by-step protocols and extensive hands-on advice.

ISBN-13

9783642477331

ISBN-10

364247733X

Weight

0.80 Pounds

Dimensions

6.10 x 0.58 x 9.25 In

List Price

$54.99

Edition

1st Edition

Format

Paperback

Language

English

Pages

xiii, 241 pages

Publisher

Springer

Published On

2012-11-06



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Bonita
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