This bookconstitutes the proceedings of the Second Australasian Conference on ArtificialLife and Computational Intelligence, ACALCI 2016, held in Canberra, ACT,Australia, in February 2016.

The 30 fullpapers presented in this volume were carefully reviewed and selected from 41submissions. They are organized in topical sections named: mathematicalmodeling and theory; learning and optimization; planning and scheduling;feature selection; and applications and games.